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1st Place, 2007

Gloria Kwon,

 Double transgenic mouse embryo, 18.5 days (17X)
Affiliation
Memorial Sloan-Kettering Insititute
Location
New York, New York, USA
Technique
Brightfield, Darkfield, Fluorescence (GFP and RFP)

Kwon’s image is a by-product of current research for her graduate thesis. It illustrates a late-stage developing mouse embryo with the yolk sac and placenta intact. All of the structures within the embryo have red fluorescence, while the yolk sac exclusively has green fluorescence, showing the details of the body plan. Her goal was to create a clean and focused photograph where the smaller details were as clear as the bigger picture.

To capture this image, Kwon used widefield microscopy with various lighting conditions under brightfield as well as green and red fluorescent filters in darkfield. Through techniques like this she has been able to visualize specific details of the developing mouse embryo that could not otherwise be observed or documented.

Nikon Instruments Inc.