2011 Small World In Motion Competition
Dr. Liang Gao
Howard Hughes Medical Institute (HHMI)
Janelia Farm Research Campus
- Subject Matter:
An African green monkey kidney cell (COS-7) transfected with mEmerald/cSrc showing membrane ruffles
Two Photon Bessel Beam Plane Illumination Microscopy
see: 2-photon, bessel beam
Q&A WITH THE WINNER
What is the subject matter of your winning entry?
Retrograde flow of membrane ruffles formed at the leading edge of an African green monkey kidney cell as well as the macropinocytosis process showing the intracellular vacuoles formation and dynamics.
Why did you choose to submit this particular movie?
This is a good looking cell showing active dynamics.
Can you elaborate on the imaging technique you used?
It’s an example of the imaging quality of the Bessel beam plane Illumination microscopy. A thin excitation light sheet created by a scanning Bessel beam allows fast imaging with excellent three dimensional spatial resolution.