Techniques

SIM

3rd Place
2013 Small World In Motion Competition

Dr. Lin Shao

Howard Hughes Medical Institute (HHMI)
Ashburn, Virginia, USA

Subject Matter:

Live HeLa (cancer) Cell Stained with MitroTracker Green

- see: HeLa Cell, Cancer Cell
Technique:

Fast 3D wide-field structured illumination microscopy (SIM)


see: SIM

Dr. Lin Shao’s video shows for the very first time the inner details of the mitochondria in a living cell within a 3D image. Shao used structured illuminated microscopy applied to the wide field microscope, doubling the normal resolution of the conventional microscope. With this technique, Dr. Shao captured more than 50 time points which were then reconstructed in three dimensions. Shao hopes this video inspires other microscopists to apply this technique in their own laboratories.