Annual Winners

2012 Small World In Motion Competition

Honorable Mention
2012 Small World In Motion Competition

Michael Weber

Max Planck Institute of Molecular Cell Biology and Genetics
Dresden, Germany

Subject Matter:

Beating heart of a living 2 day old Danio rerio (zebrafish)

- see: Zebrafish (20x)
Technique:

Selective Plane Illumination Microscopy (SPIM)


The heart muscle cells of this transgenic fish expresses GCaMP, a genetically encoded fluorescent calcium indicator. Visible is the wave of cardiac conduction, traveling over the heart from atrium (bottom) to ventricle (top left). The heart has a size of about 250 um and beats at a rate of about two times per second. It was imaged in 3D inside the living zebrafish using Selective Plane Illumination Microscopy (SPIM) (Huisken et al. 2004).