A sheet of light is used to illuminate the plant (Arabidopsis thaliana), from the side while collecting the emitted light at a perpendicular axis. The plant grows in an upright position in the microscope’s specimen chamber. While the leaves remain in the air, the root system is perfused with liquid medium. and root stably expresses the early endosome/exosome marker 35S::GFP-RabA1d. The quick endosomes move with up to 10 µm/s, which presents a serious imaging challenge. This technique allows new insides in the dynamics of endosomal compartments in plant cells. The Arabidopsis line was kindly provided by Tobias Berson and Jozef Samaj.
2012 Small World In Motion Competition

Honorable Mention
Fast moving endosomes in Arabidopsis thaliana root cells
Dr. Daniel von Wangenheim
- Affiliation
- Goethe University
BMLS - Buchmann Institute for Molecular Life Sciences
Frankfurt am Main, Germany
- Technique
- Light Sheet-based Fluorescence Microscopy
- Magnification
- 63x
Top 20
1st

Olena Kamenyeva
2nd

Stefan Lüpold
3rd

Nils Lindström
Honorable Mentions
HM

Andrew Dopheide
HM

Oleg Lavrentovich
HM

Kathryn Markey
HM

Maria Nemethova
HM

Phuong Anh Nguyen
HM

Heiti Paves
HM

Wim van Egmond
HM

Daniel von Wangenheim
HM

Michael Weber
HM

Fengzhu Xiong