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Pin-ItSperm of two different males (green and red) competing within the female reproductive tract of the fruit fly Drosophila melanogaster. Competition between sperm is a widespread phenomenon throughout the animal kingdom and a powerful evolutionary force driving species diversity. However, it has been nearly impossible to study the fundamental biological processes associated with such sperm competition, occurring whenever sperm from different males mix inside of females. The very recent development of genetically modified fruit flies that produce sperm with either green- or red-fluorescent heads (as seen in the movie) is now allowing us to answer important biological questions.
2012 Small World In Motion Competition
2nd Place
Sperm from two males competing within reproductive tract of a female fruit fly (Drosophila melanogaster)
Dr. Stefan Lüpold
- Affiliation
- Syracuse University
Department of Biology
Syracuse, New York, USA
- Technique
- Fluorescence
- Magnification
- 400x
1st Place
Recruitment of neutrophils to the site of laser damage in mouse inguinal lymph node
Dr. Olena Kamenyeva
- Affiliation
- National Institutes of Health (NIH)
NIAID - Laboratory of Immunoregulation
Bethesda, Maryland, USA
- Technique
- 2-photon
This video shows the immune response in the lymph node of a mouse, when activated by a laser. Specifically, it shows an efficient innate immune reaction in the lymph node, which typically has been studied for the development of adaptive immune response.
3rd Place
Complexity of ureteric bud branching and nephron formation
Dr. Nils Lindström
- Affiliation
- University of Edinburgh
The Roslin Institute
Edinburgh, Midlothian, United Kingdom
- Technique
- Time-lapse, inverted fluorescent microscope, organ culture, transgenic mouse reporter lines
Illustrating the development of live kidney cells, specifically a metanephric kidney that has been cultured in vitro and imaged over 4 days. The fluorescence originates from a conditional YFP reporter, which is only activated in cells expressing Pax8. The fluorescent cells belong to the ureteric bud (the tree), the induced nephron progenitors cell (cells around the tree tips) and nephrons that are forming (the shapes forming within the tree). The YFP signal is viewed using a heat-map that has been overlaid onto the bright-field channel.
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