2012 Small World In Motion Competition

Limnias melicerta (a rotifer)

Wim van Egmond

Affiliation
Micropolitan Museum
Berkel en Rodenrijs, Zuid-Holland, The Netherlands
Technique
Differential Interference Contrast
Magnification
200x

This microanimal lives in a self-built tube attached to waterplants. We see the rotifer using fast moving cilia to create a vortex. This enables it to sweep in food particles like algae. Inside the organism we can also see jaw-like structures that grind the food.

Movement of organelles in plant cells (onion bulb scale epidermis)

Dr. Heiti Paves

Affiliation
Tallinn University of Technology
Tallinn, Estonia
Technique
Differential Interference Contrast
Magnification
20x

Fast moving endosomes in Arabidopsis thaliana root cells

Dr. Daniel von Wangenheim

Affiliation
Goethe University
BMLS - Buchmann Institute for Molecular Life Sciences
Frankfurt am Main, Germany
Technique
Light Sheet-based Fluorescence Microscopy
Magnification
63x

A sheet of light is used to illuminate the plant (Arabidopsis thaliana), from the side while collecting the emitted light at a perpendicular axis. The plant grows in an upright position in the microscope’s specimen chamber.  While the leaves remain in the air, the root system is perfused with liquid medium. and root stably expresses the early endosome/exosome marker 35S::GFP-RabA1d. The quick endosomes move with up to 10 µm/s, which presents a serious imaging challenge. This technique allows new insides in the dynamics of endosomal compartments in plant cells. The Arabidopsis line was kindly provided by Tobias Berson and Jozef Samaj.