2020 Small World in Motion Competition

The first 22 hours of zebrafish development (blood vessels shown in green)

Daniel Castranova Dr. Brant M. Weinstein

Affiliation
National Institutes of Health (NIH)
NICHD
Bethesda, Maryland, USA
Technique
Confocal
Magnifaction
4X (Objective Lens Magnification)

Daniel Castranova, a research technician at the NIH in Bethesda MD, and the Nikon Small World first place winner in the still imaging competition (Daniel is having a VERY good year) took this image of the first 22 hours of the development of a developing transgenic zebrafish embryo. This movie combined transmitted light and confocal microscopies to beautifully illustrate the blood vessels developing from a single cell. He says that “keeping a fertilized egg alive, happy and oriented properly throughout early development requires both luck and skill” however, we think the luck part bears a strong correlation to the skill part.