2020 Photomicrography Competition

Embryonic development of a clownfish (Amphiprion percula) on days 1, 3 (morning and evening), 5, and 9

Daniel Knop

Affiliation
Natur und Tier-Verlag NTV
Oberzent-Airlenbach, Hessen, Germany
Technique
Image Stacking
Magnifaction
10X (Objective Lens Magnification)

Daniel Knop's image of the embryonic development of a clownfish (Amphiprion percula) on days 1, 3 (morning and evening), 5, and 9, was created using image-stacking. It shows the development, from hours after fertilization (even with a pack of sperm cells being visible on top of the egg), until hours before hatching. The primary challenge was to create sharp focus stacking pictures while the embryo was alive and moving.