News

Rodent Optic Nerve Head Wins the 49th Annual Nikon Small World Photo Microscopy Competition

Nikon Instruments Inc. today unveiled the winners of the 49th annual Nikon Small World Photomicrography Competition. This year’s first place prize was awarded to Hassanain Qambari, assisted by Jayden Dickson of the Lions Eye Institute, for his vivid image of a rodent optic nerve head showing astrocytes (yellow), contractile proteins (red), and retinal vasculature (green). The colorful image provides an important contribution to the study and reversal of diabetic retinopathy, which affects one in five persons with diabetes worldwide.

Diabetic retinopathy occurs when high blood sugar damages the blood vessels in the tissue at the back of the eye, known as the retina. The damaged blood vessels can swell and leak, which can cause blurry vision or total loss of eyesight. Since 2021, Qambari has devoted his time and research to the early detection and reversal of the disease.

“Current diagnostic criteria and treatment regimens for diabetic retinopathy are limited to the late-stage appearance of the disease, with irreversible damage to retinal microvasculature and function,” said Qambari. “The visual system is a complex and highly specialized organ, with even relatively minor perturbations to the retinal circulation able to cause devastating vision loss. I entered the competition as a way to showcase the complexity of retinal microcirculation.”

Qambari faced some challenges when capturing his image such as locating fine vessels near 110 microns in diameter and establishing a protocol for labeling different cell types. “Over the past 20 years, our research group has refined the technique of isolated ocular perfusion labeling for fine vessels in the eye,” said Qambari. “While the ophthalmic artery in the rodent model presented a technically demanding challenge, we were able to overcome it with persistence and patience.”

The final result educates the public about the universal condition affecting millions and the vital research necessary to further advance care. “The Nikon Small World competition is great, as it showcases amazing work across many disciplines from around the world,” said Qambari. “All the images presented in the competition represent the beauty and artistic side of science which may otherwise get overlooked. Such a competition not only celebrates the participant's hard work and passion but may also draw and inspire young scientists to pursue a career in STEM. It certainly inspired me.”

Like Qambari, Eric Flem, Senior Manager, CRM and Communications at Nikon Instruments, is passionate about sharing exemplary scientific work and artistic techniques. “The past 49 years of this competition have borne witness to many innovative and pioneering advancements in scientific imaging technology,” said Flem. “I am consistently awed by how these advancements make it possible to create art out of science for the public to enjoy.”

Second place was awarded to Ole Bielfeldt for his image of a matchstick igniting by the friction surface of the box. The image was taken within one eight-thousands of a second and utilized imaging stacking.

Third place was awarded to Malgorzata Lisowska for her image of breast cancer cells.

In addition to the top three winners, Nikon Small World recognized 83 photos out of thousands of entries from scientists and artists across the globe.

The 2023 judging panel included:

  • Ed Cara, Science and Health Reporter at Gizmodo
  • James Cutmore, Picture Editor at BBC Science Focus Magazine
  • Dr. Gary Laevsky, Director of the Confocal Imaging Facility at Princeton University
  • Dr. Igor Siwanowicz, Research Scientist at Howard Hughes Medical Institute
  • Dr. Clare Waterman, Cell Biologist and Member of the National Academy of Sciences

NIKON SMALL WORLD WINNERS

1st Place
Hassanain Qambari & Jayden Dickson

The Lions Eye Institute
Department of Physiology & Pharmacology
Perth, Western Australia, Australia
Rodent optic nerve head showing astrocytes (yellow), contractile proteins (red) and retinal vasculature (green)

Confocal, Fluorescence, Image Stacking
20X (Objective Lens Magnification)

2nd Place
Ole Bielfeldt

Macrofying
Cologne, North Rhine-Westphalia, Germany
Matchstick igniting by the friction surface of the box.

Brightfield, Image Stacking
2.5X (Objective Lens Magnification)

3rd Place
Malgorzata Lisowska

Independent Value Based Healthcare Consultant
Warsaw, Mazowieckie, Poland
Breast cancer cells

Brightfield, Image Stacking
40X (Objective Lens Magnification)

4th Place
John-Oliver Dum

Medienbunker Produktion
Bendorf, Rheinland-Pfalz, Germany
Venomous fangs of a small tarantula

Image Stacking
10X (Objective Lens Magnification)

5th Place
Dr. David Maitland

www.davidmaitland.com
Feltwell, Norfolk, United Kingdom
Auto-fluorescing defensive hairs covering the leaf surface of Eleagnus angustifolia exposed to UV light

Fluorescence, Image Stacking
10X (Objective Lens Magnification)

6th Place
Timothy Boomer

WildMacro.com
Vacaville, California, USA
Slime mold (Comatricha nigra) showing capillitial fibers through its translucent peridium

Image Stacking
10X (Objective Lens Magnification)

7th Place
Dr. Grigorii Timin & Dr. Michel Milinkovitch

University of Geneva
Department of Genetics and Evolution
Geneva, Switzerland
Mouse embryo

Light Sheet
4X (Objective Lens Magnification)

8th Place
Stefan Eberhard

University of Georgia
Athens, Georgia, USA
Caffeine crystals

Polarized Light
25X (Objective Lens Magnification)

9th Place
Vaibhav Deshmukh

Baylor College of Medicine
Department of Molecular Physiology and Biophysics
Houston, Texas, USA
Cytoskeleton of a dividing myoblast; tubulin (cyan), F-actin (orange) and nucleus (magenta)

Fluorescence, Structured Illumination Microscopy (SIM)
63X (Objective Lens Magnification)

10th Place
Melinda Beccari & Dr. Don W. Cleveland

UC San Diego
Department of Cellular and Molecular Medicine
La Jolla, California, USA
Motor neurons grown in microfluidic device for separation of cell bodies (top) and axons (bottom). Green - microtubules; Red - growth cones (actin).
Confocal, Fluorescence
20X (Objective Lens Magnification)

11th Place
Dr. Diego García

Universidad Complutense de Madrid
Real Sociedad Española de Física
Madrid, Spain
Crystallized sugar syrup

Polarized Light
25X (Objective Lens Magnification)

12th Place
Sherif Abdallah Ahmed

Faculty of Science, Tanta University
Department of Zoology
Tanta, Egypt, Arab Republic
"Cuckoo wasp" standing on a flower

Image Stacking
4X (Objective Lens Magnification)

13th Place
Satu Paavonsalo & Dr. Sinem Karaman

University of Helsinki
Individualized Drug Therapy Research Program, Faculty of Medicine
Helsinki, Finland
Blood and lymphatic vasculatures in the ear skin of an adult mouse

Confocal
10X (Objective Lens Magnification)

14th Place
John-Oliver Dum

Medienbunker Produktion
Bendorf, Rheinland-Pfalz, Germany
Sunflower pollen on an acupuncture needle

Image Stacking
40X (Objective Lens Magnification)

15th Place
Dr. Pichaya Lertvilai

UC San Diego
Scripps Institution of Oceanography
La Jolla, California, USA
Fluorescent image of an Acropora sp. showing individual polyps with symbiotic zooxanthellae

Darkfield, Fluorescence, Image Stacking
5X (Objective Lens Magnification)

16th Place
Dr. Diego García

Universidad Complutense de Madrid
Real Sociedad Española de Física
Madrid, Spain
Carbon nanotubes

Stereomicroscopy
30X (Objective Lens Magnification)

17th Place
Yuan Ji

World Expo Museum
Shanghai, China
Chinese moon moth (Actias ningpoana) wing scales

Image Stacking
20X (Objective Lens Magnification)

18th Place
Scott Peterson

New Hope, Minnesota, USA
A cryptocrystalline micrometeorite resting on a #80 testing sieve.

Image Stacking
20X (Objective Lens Magnification)

19th Place
Marek Miś

Marek Miś Photography
Suwalki, Podlaskie, Poland
Stomata in peace lily (Spathiphyllum sp.) leaf epidermis

Polarized Light
40X (Objective Lens Magnification)

20th Place
Daniel Castranova & Dr. Brant Weinstein

National Institutes of Health (NIH), Eunice Kennedy Shriver National Institute of Child Health and Human Development
Bethesda, Maryland, USA
Adult transgenic zebrafish head showing blood vessels (blue), lymphatic vessels (yellow), and the skin and scales (magenta)

Confocal
4X (Objective Lens Magnification)

HM
Dr. Arthur Chien & Dr. Ann Na Cho

Macquarie University
Microscopy Facility
Macquarie University, New South Wales, Australia
Organ-on-chip system enabling the synaptic conjugation between 3D human embryonic stem cells

Confocal
10X (Objective Lens Magnification)

HM
Dr. Amy Engevik

Medical University of South Carolina
Department of Regenerative Medicine & Cell Biology
Charleston, South Carolina, USA
Neonatal mouse intestinal tissue cells

Fluorescence
20X (Objective Lens Magnification)

HM
Dr. Nathan P. Myhrvold

Modernist Cuisine
Bellevue, Washington, USA
Trichinella cyst in pork muscle (Trichinella is a parasitic worm known to cause trichinosis)

Brightfield, Image Stacking
10X (Objective Lens Magnification)

HM
Ángel Navarro Gómez

Madrid, Spain
Carpenter bee (Xylocopa violacea) head and antenna

Image Stacking
20X (Objective Lens Magnification)

HM
Dr. Andrew M. Posselt

University of California, San Francisco (UCSF)
Division of Transplant Surgery
Mill Valley, California, USA
Underside of cellar spider (Pholcus phalangioides)

Image Stacking
10X (Objective Lens Magnification)

HM
Dr. Grigorii Timin & Dr. Michel Milinkovitch

University of Geneva
Department of Genetics and Evolution
Geneva, Switzerland
Dermal collagen in embryonic snake scales

Confocal
63X (Objective Lens Magnification)

HM
Dr. Bas van Bommel

Freie Universität Berlin
Department of Biochemistry
Berlin, Germany
Rat astrocytes

Confocal
40X (Objective Lens Magnification)

HM
Travis Wagner

Rochester Institute of Technology
Department of Mechanical Engineering
Rochester, New York, USA
Sphagnum moss with two air bubbles on the sample

Fluorescence, Image Stacking
20X (Objective Lens Magnification)

IoD
Dr. Florian Alonso

University of Bordeaux
BioTis-INSERM U1026
Pessac, Gironde, France
Formation of blood vessels (angiogenesis) in the retina from a Lifeact-EGFP newborn mouse

Confocal
63X (Objective Lens Magnification)

IoD
Raghuram Annadana

Raghuram Annadana Photography
Bangalore, Karnataka, India
Developing stamen and stigma inside a Hibiscus flower bud

Panoramic Image Stacking
10X (Objective Lens Magnification)

IoD
Dr. Alexandre Beber

Institute of Biotechnology CAS
Vestec, Central Bohemia, Czech Republic
Fluorescent actin filaments (yellow) and fluorescent anillin protein (blue) deposited on a glass coverslip after dewetting

Fluorescence, Confocal
60X (Objective Lens Magnification)

IoD
Todd Becker

Teabeck
Verona, Wisconsin, USA
Platinum spark plug

Image Stacking, Reflected Light
4X (Objective Lens Magnification)

IoD
Dr. Frantisek Bednar

Svosov, Zilinsky, Slovak Republic
Slime mold (Trichia crateriformis)

Image Stacking
5X (Objective Lens Magnification)

IoD
Taylor Bell

Gustometry + SF Micro Society
Norwalk, Connecticut, USA
Freshwater amphipod

Image Stacking
4X (Objective Lens Magnification)

IoD
Timothy Boomer

WildMacro.com
Vacaville, California, USA
Slime mold (Didymium sp.) fruiting bodies

Image Stacking
10X (Objective Lens Magnification)

IoD
Dr. Michael John Bridge & Michael Sieverts

University of Utah
HSC Cell Imaging Core
Salt Lake City, Utah, USA
Mouse femur bone lacunar-canalicular network (voids in bone that house osteocytes and their interconnected micro-tubular processes)

Confocal
63X (Objective Lens Magnification)

IoD
Arturo Calderón, Dr. Miguel Tapia-Rodríguez & Dr. Juan Pedro Laclette

National Autonomous University of Mexico (UNAM)
Department of Immunology
Mexico City, Mexico
Muscle architecture of an evaginating tapeworm (Taenia crassiceps cysticercus)

Confocal, Fluorescence, Image Stacking
10X (Objective Lens Magnification)

IoD
Hsuan Chen

Academia Sinica
Chen-Hui Chen’s Lab in the Institute of Cellular and Organismic Biology
Taipei City, Taipei, Taiwan
In toto
image of the skin and mucous cells in a live zebrafish larva

Confocal
10X (Objective Lens Magnification)

IoD
Dr. Arthur Chien

Macquarie University
Microscopy Facility
Macquarie University, New South Wales, Australia
Cleared mouse embryo

Stereomicroscopy, Reflected Light, Focus Stacking
0.5X (Objective Lens Magnification)

IoD
Nikky Corthout & Alex Calzoni

VIB (Flanders Institute of Biotechnology)
Center for Brain and Disease Research
Leuven, Vlaams-Brabant, Belgium
Retrograde labeled neurons in the cortex of a cortical mouse brain section

Image Stacking, Fluorescence, Confocal
20X (Objective Lens Magnification)

IoD
John-Oliver Dum

Medienbunker Produktion
Bendorf, Rheinland-Pfalz, Germany
Cabbage butterfly eggs

Brightfield, Image Stacking
20X (Objective Lens Magnification)

IoD
Nadia Efimova

Amicus Therapeutics
Philadelphia, Pennsylvania, USA
Maturing mouse cortical neuron in culture

Confocal, Deconvolution, Fluorescence
63X (Objective Lens Magnification)

IoD
Ricardo Roberto Fernández Martínez

IES Virgen de la Luz
Department of Biology and Geology
Avilés, Asturias, Spain
Tail of planktonic shrimp larvae

Image Stacking
17X (Objective Lens Magnification)

IoD
Frank Fox

Trier University of Applied Sciences
Konz, Rheinland-Pfalz, Germany
Marine organism (Pyrocystis lunula, Dinophyceae)

Differential Interference Contrast (DIC)
100X (Objective Lens Magnification)

IoD
Ian Gardiner

Calgary, Alberta, Canada
Clam shrimp (Lynceus mucronatus)

Reflected Light, Focus Stacking
2.5X (Objective Lens Magnification)

IoD
Dr. Saikat Ghosh & Dr. Juan S. Bonifacino

National Institutes of Health
NICHD
Bethesda, Maryland, USA
iPSC-derived human neurons

Confocal
40X (Objective Lens Magnification)

IoD
Dr. Thomas G.W. Graham

University of California, Berkeley
Department of Molecular and Cell Biology
Berkeley, California, USA
Algae from a mud puddle

Confocal
10X (Objective Lens Magnification)

IoD
Daniel B. Hoffman & Dr. Jacob Sorensen

University of Minnesota
Department of Kinesiology
Minneapolis, Minnesota, USA
Rat skeletal muscle fibers with associated neuromuscular junctions (white)

Confocal
40X (Objective Lens Magnification)

IoD
Dr. Hema Saranya Ilamathi

Université du Québec à Trois-Rivières (UQTR)
Department of Medical biology
Trois-Rivieres, Quebec, Canada
Distribution of cellular batteries (mitochondria-yellow), along the transport cables (Tubulin-red, actin-cyan) in human fibroblast

Confocal
63X (Objective Lens Magnification)

IoD
Don Komarechka

Komarechka Photography Ltd.
Ravna Gora, Varna, Bulgaria
Two fluorescing diamonds

Fluorescence
10X (Objective Lens Magnification)

IoD
Charles Krebs

Charles Krebs Photography
Issaquah, Washington, USA
Feeding bryozoan colony zooids. Bryozoans are microscopic aquatic invertebrates that live in colonies.

Reflected Light
5X (Objective Lens Magnification)

IoD
Charles Krebs

Charles Krebs Photography
Issaquah, Washington, USA
Mushroom gills showing sporophores (sporangiophores)

Darkfield, Image Stacking
20X (Objective Lens Magnification)

IoD
Dr. Håkan Kvarnström

Bromma, Sweden
Amoeba (Arcella)

Differential Interference Contrast (DIC)
60X (Objective Lens Magnification)

IoD
Michael Landgrebe

Weissensberg, Bavaria, Germany
Fossil diatom

Brightfield (inverted)
60X (Objective Lens Magnification)

IoD
Dr. Francisco Lázaro-Diéguez

Albert Einstein College of Medicine
Bronx, New York, USA
Non-parenchymal liver cells

Confocal
63X (Objective Lens Magnification)

IoD
Dr. Olivier Leroux

Ghent University
Faculty of Sciences/Faculty of Bioscience Engineering
Gent, Oost-Vlaanderen, Belgium
Composition of transverse sections of plant organs

Fluorescence
20X (Objective Lens Magnification)

IoD
Dr. Pichaya Lertvilai

University of California San Diego
Scripps Institution of Oceanography
La Jolla, California, USA
Coral (Acropora granulosa) fluorescing under blue light

Image Stacking, Fluorescence, Darkfield
5X (Objective Lens Magnification)

IoD
Dr. Yu-Hsiu Liu

Academia Sinica
Chen-Hui Chen's Lab in the Institute of Cellular and Organismic Biology
Nankang, Taipei, Taiwan
Palmskin zebrafish larva

Confocal
25X (Objective Lens Magnification)

IoD
Walter Machielsen

www.waltermachielsen.com
Rotterdam, Zuid-Holland, Netherlands
Buckthorn trichomes

Differential Interference Contrast (DIC)
40X (Objective Lens Magnification)

IoD
Dr. David Maitland

www.davidmaitland.com
Feltwell, Norfolk, United Kingdom
Wing scales of the cinnabar moth (Tyria jacobaeae) under ultraviolet light (UV)

Fluorescence, Image Stacking
20X (Objective Lens Magnification)

IoD
Sébastien Malo

Saint Lys, Haute-Garonne, France
Crab spider (Thomisus onustus)

Darkfield, Image Stacking, Reflected Light
6.3X (Objective Lens Magnification)

IoD
Sébastien Malo

Saint Lys, Haute-Garonne, France
Geranium (Geraniaceae) stamen covered in pollen

Darkfield, Image Stacking, Reflected Light
6.3X (Objective Lens Magnification)

IoD
Amir Maqbool

Higher Education Department Jammu and Kashmir India
Department of Zoology
Srinagar, Jammu and Kashmir, India
Phoretic mites on the leg of a bumblebee

Image Stacking
3X (Objective Lens Magnification)

IoD
Dr. Robert Markus

University of Nottingham
School of Life Sciences Imaging (SLIM)
Nottingham, Nottinghamshire, United Kingdom
Actin cytoskeleton of bovine pulmonary epithelial cells

Structured Illumination Microscopy (SIM)
63X (Objective Lens Magnification)

IoD
Dr. Simon Frederik Merz, Dr. Lea Bornemann & Dr. Ewa Patrycja Smajek

LaVision BioTec, a Miltenyi Biotec Company
Department of Biophysics
Bielefeld, North-Rhine Westphalia, Germany
Fly (cyan) caught in a Venus flytrap (red)

Fluorescence, Light Sheet
2.4X (Objective Lens Magnification)

IoD
Marek Miś

Marek Miś Photography
Suwalki, Podlaskie, Poland
Leaf epidermis stomata (Stromanthe sp.)

Polarized Light
10X (Objective Lens Magnification)

IoD
Ángel Navarro Gómez

Madrid, Spain
Mechanosensors in a Venus flytrap

Image Stacking
20X (Objective Lens Magnification)

IoD
Dr. Foo Yong Ng

Faculty of Science and Technology, Universiti Kebangsaan Malaysia
Department of Biology Science and Biotechnology
Bangi, Selangot, Malaysia
Moss

Image Stacking
2X (Objective Lens Magnification)

IoD
Dr. Lori O'Brien

University of North Carolina at Chapel Hill
Department of Cell Biology and Physiology
Chapel Hill, North Carolina, USA
Embryonic mouse (Mus musculus) kidney showing the collecting duct (blue) and nephron progenitor (yellow) cells

Confocal
5X (Objective Lens Magnification)

IoD
Yusuf Ziya Öztürk

TCDD Teknik Müh. Müş. A.Ş.
Ankara, Çankaya, Turkey
Bee

Image Stacking
3.7X (Objective Lens Magnification)

IoD
Alison Pollack

San Anselmo, California, USA
Slime mold (Craterium leucocephalum), looking like a beautiful tiny goblet

Image Stacking, Reflected Light
10X (Objective Lens Magnification)

IoD
Alison Pollack

San Anselmo, California, USA
Slime mold (Diderma tigrinum)

Image Stacking, Reflected Light
10X (Objective Lens Magnification)

IoD
Dr. Andrew M. Posselt

University of California, San Francisco (UCSF)
Division of Transplant Surgery
Mill Valley, California, USA
Blue black weevil (Metapocyrtus sp.)

Image Stacking
6X (Objective Lens Magnification)

IoD
Frank Reiser

Nassau Community College
Department of Biology
Garden City, New York, USA
Ostracods and algae (Cladophora)

Darkfield, Reflected Light
2.5X (Objective Lens Magnification)

IoD
Dr. Colin Rogers & Dr. Erica Weekman

University of Kentucky
Sanders Brown Center on Aging
Lexington, Kentucky, USA
Mouse retina blood vessels (green) astrocytes (red) and microglia (purple)

Confocal
20X (Objective Lens Magnification)

IoD
Jan Rosenboom

Rostock, Mecklenburg Vorpommern, Germany
Diatoms (single-celled algae) arranged on the head of a pin

Image Stacking, Reflected Light
4X (Objective Lens Magnification)

IoD
Danny Sanchez

Mineralien LLC
Valley Village, California, USA
Golden rutile in quartz

Reflected Light, Darkfield
6X (Objective Lens Magnification)

IoD
Hans Schoofs

Uppsala University
Department of Immunology, Genetics and Pathology (IGP)
Uppsala, Sweden
Blood and lymphatic vessels in a mouse diaphragm

Confocal
20X (Objective Lens Magnification)

IoD
Dr. Leo Serra

University of Cambridge
Sainsbury Laboratory
Cambridge, Cambridgeshire, United Kingdom
Bindweed (Convolvulus) leaf epidermal cells autofluorescing

Confocal
20X (Objective Lens Magnification)

IoD
Dr. Leo Serra

University of Cambridge
Sainsbury Laboratory
Cambridge, Cambridgeshire, United Kingdom
Patterns at the surface of an embryonic leaf of Thale cress (Arabidopsis thaliana)

Confocal
20X (Objective Lens Magnification)

IoD
Stephen Vidman & Dr. Andrea Tedeschi

Ohio State University
Department of Neuroscience
Columbus, Ohio, USA
3D capillary network section of the mammalian brain (dentate gyrus of the hippocampus)

Confocal, Deconvolution
10X (Objective Lens Magnification)

IoD
Priscilla Vieto Bonilla & Brandon Antonio Segura Torres

Universidad Nacional del Comahue
Department of Biological Sciences
San Carlos de Bariloche, Río Negro, Argentina
One-week-old Axolotl after hatching

Image Stacking, Stereomicroscopy
25X (Objective Lens Magnification)

IoD
Cagri Yalcin

Impressions Microscopiques
Amsterdam, Noord Holland, Netherlands
Crystals of malonic acid dissolved in ethanol

Polarized Light
4X (Objective Lens Magnification)

IoD
Zhao Zengchao

Gaush Meditech Ltd.
Hefei, Anhui Province, China
Bristle of a millipede (Polyxenidae)

Image Stacking
50X (Objective Lens Magnification)

IoD
Dr. Tong Zhang

Northwestern University
Biological Imaging Facility
Evanston, Illinois, USA
Lily (Lilium) anther cross section with pollen

Confocal
20X (Objective Lens Magnification)

IoD
Dr. Tong Zhang

Northwestern University
Biological Imaging Facility
Evanston, Illinois, USA
Lily (Lilium) anther cross section with pollen

Confocal
20X (Objective Lens Magnification)

To view all the winners, click here.