2020 Small World in Motion Competition

A stentor (ciliate) juggling green algae

Frank Fox

Affiliation
Trier University of Applied Sciences
Konz, Rheinland-Pfalz, Germany
Technique
Darkfield
Magnification
20X (Objective Lens Magnification)

One of two honorable mentions for Frank Fox in the 2020 Small World in Motion competition, this movie shows a stentor (ciliate) feeding (and perhaps playing) with green algae using darkfield microscope technique. This is not frequently observed and can be especially difficult since great care and skill is needed to assure the coverslip on the microscope slide does not interfere with the natural movements of the subject.

Cancer cell metastasis in a developing zebrafish embryo

Dr. Stephan Daetwyler Dr. Gloria Slattum, Dr. Jody Rosenblatt, Dr. Jan Huisken

Affiliation
UT Southwestern
Dallas, Texas, USA
Technique
Light Sheet
Magnification
10X (Objective Lens Magnification)

Dr. Daetwyler, a researcher at UT Southwestern Medical Center and his collaborator, Dr. Gloria Slattum focus their work on epithelial cell biology. Their main interest in creation of this movie was a visualization and further understanding of dynamic processes in the development of disease, particularly cancer. In short, this movie is part of life-saving research to learn how cancer metastasis takes place. It illustrates how mutated genes in the zebrafish epidermis cause cancerous cells to be extruded and invade surrounding tissue. Imaging events such as this require cutting edge technology that calls on the latest developments in imaging, microscopy, sample care/preparation and data processing. These movies are often derived from data sets that are measured in terabytes (1 terabyte=1,000 Gigabytes) Cancer metastasis is the primary cause of cancer associated death. The knowledge gained from such research will translate directly to more effective ways of controlling this deadly aspect of the disease.

Hydra

Frank Fox

Affiliation
Trier University of Applied Sciences
Konz, Rheinland-Pfalz, Germany
Technique
Darkfield
Magnification
10X (Objective Lens Magnification)

Frank Fox’s second honorable mention in the 2020 Small World in Motion competition shows a Hydra, an invertebrate freshwater animal that is known for being able to expand its normal size. This video shows just such behavior as the creature stretches out many times its original length. This was taken using darkfield technique and clearly demonstrates Mr. Fox’s ability to keep moving subjects in focus, a challenge for even the most accomplished microscopist.